Discriminative Metabolomics Analysis and Cytotoxic Evaluation of Flowers, Leaves, and Roots Extracts of Matthiola longipetala subsp. livida

Matthiola longipetala subsp. livida is an annual herb in Brassicaceae that has received little attention despite the family’s high reputation for health benefits, particularly cancer prevention. In this study, UPLC-HRMS-MS analysis was used mapping chemical constituents of different plant parts (i.e., flowers, leaves, and roots). Also, spectral similarity networks via Global Natural Products Social Molecular Networking (GNPS) were employed to visualize their differences similarities. Additionally, cytotoxic activity on HCT-116, HeLa, HepG2 cell lines evaluated. Throughout current analysis, 154 compounds annotated, with prevalence phenolic acids, glucosinolates, flavonol glucosides, lipids, peptides, others. Predictably, secondary metabolites (phenolic flavonoids, glucosinolates) predominant flowers while roots characterized by primary (peptides fatty acids). Four diacetyl derivatives tentatively assigned as O-acetyl O-malonyl glucoside quercetin (103), kaempferol (108 112), isorhamnetin (114) detected first time nature. The leaves extracts showed significant inhibition HeLa line propagation LC50 values 18.1 ± 0.42 29.6 0.35 µg/mL, respectively, whereas extract inhibited HCT-116 24.8 0.45 compared those Doxorubicin (26.1 0.27 37.6 0.21 µg/mL), respectively. conclusion, M. are responsible abundance bioactive properties.